We know from statements and observations that pine marten were in Somerset in the 1990s and may well have been some of the released pairs.
DNA samples from elusive pine martens are being collected as part of a study in the New Forest.
The rare mammals are now believed to be well-established and successfully breeding in the national park.
The elusive cat-sized member of the weasel family was previously only thought to have survived largely in the north of England.
Researchers are collecting genetic material in order to establish the health of the population in the forest.
Hidden cameras have shown young pine martens exploring and playing together over a number of years.
The footage was shot as part of a study aiming to determine if and how pine martens were recolonising the area.
Marcus Ward of Wild New Forest said the latest phase, collecting hair samples using pipes lined with velcro, would provide an "insight" into the forest's pine martens.
"The main thing we are looking at is the genetic diversity of the population and how healthy the population is," he said.
"By getting DNA, it unlocks other bits of information which is useful and helps us piece together our understanding of pine martens in the New Forest."
The video footage showed pine martens prefer to use fallen trees and branches to navigate across the forest floor and to cross streams and wet areas.
The creatures sleep and make dens high up in the trees and usually only come out at night to hunt.
The pine marten study is being carried out by Wild New Forest along with Forestry England, The New Forest Study Group, Hampshire and Isle of Wight Wildlife Trust.
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